СD95-DEFICIENT CELLS OF JURKAT/A4 SUBLINE ARE RESISTANT TO DRUG-INDUCED APOPTOSIS

Sokolovskaya A.A., Zabotina T.N., Blokhin D.Yu., Inshakov A.N., Mikhailov A.D., Kadagidze Z.G., Baryshnikov A.Yu.

We developed unique CD95-deficient subline (Jurkat/A4) from the parent Jurkat/WT cell line by continuous culture in medium containing apoptosis-inducing anti-CD95 MAb, clone IPO-4 (IgM). In contrast to the parental Jurkat/WT line, which displays relatively high expression of CD95 (60–80% antigene-positive cells), cell-surface expression of the CD95 was entirely negative in the Jurkat/A4 cell line (only 4% of antigene-positive cells). The Jurkat/A4 cells are resistant to induction of apoptosis by both anti-CD95 mAb IPO-4 and chemotherapeutic drugs, whereas the wild type clone Jurkat/WT undergoes time-dependent apoptosis. Thus, these results suggest the possible participation of CD95 pathway in the development of drug-induced apoptosis.

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