AUTOOSCILLATION OF TRANSCRIPTION LEVEL AND NUCLEAR VOLUME INDUCED IN THE CELLS OF EHRLICH CARCINOMA, NK/LY LYMPHOMA, L-1210 AND P-388 LEUKEMIA BY PARTIAL BLOKADE OF RNA SYNTHESIS WITH ACTINOMYCIN D
Upon partial (50–90%) inhibition of RNA synthesis with actinomycin D with the following removal of unbinded antibiotic the dynamics of RNA synthesis in the tumor cells is changing: regular alterations in transcription rate appear and the process acquires a character of autooscillation. The amplitude and frequency of oscillations (1–3 oscillation per hour) depend on the concentration of antibiotic. During 1 oscillation the transcription rate changes from that in control probe to complete stoppage of the process. Simultaneously with alterations in the rate of RNA synthesis the alterations in the size of cell nuclei are registered (with amplitude around 30% of the medium volume). The nuclei have minimal volume during the active transcription and the maximal volume – in the period when RNA synthesis is stopped.
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