A SINGLE NUCLEOTIDE POLYMORPHISM IN THE MATRIX METALLOPROTEINASE-1 GENE PROMOTER IN COLORECTAL CANCER
Matrix metalloproteinase-1 (MMP-1) degrades collagens type I, II, and III, the main components of the interstitial stroma. Overexpression of MMP-1 was observed in various cancers and was shown to be correlated with the venous invasion, lymph node metastasis and a poor prognosis in colorectal cancer. A guanine insertion/deletion polymorphism (the 1G/2G polymorphism) within the promoter region of MMP-1 gene influences its transcription level — the 2G (insertion type) promoter has higher transcriptional activity than the 1G (deletion type) promoter. In the present work the distribution of genotypes and frequency of alleles of the 1G/2G polymorphism in subjects with colorectal cancer were investigated. Tumor tissues and distant mucosa samples were obtained from 33 patients. Blood samples from 52 sex and age matched healthy persons served as control. The 1G/2G polymorphism was determined by PCR-based XmnI restriction fragment length polymorphism. No differences between genotypes of the 1G/2G polymorphism in cancer tissue and distant mucosa were found. The distributions of the genotypes in cancer patients and control did not differ significantly (p > 0.05) from those predicted by the Hardy–Weinberg distribution. There were no differences in the frequencies of the 1G and 2G alleles between both groups. Our results suggests that the 1G/2G polymorphism may not be associated with colorectal cancer but further research performed on larger population is needed to clarify this point.
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